Turkesterone, USES and production process dew hydrophytes (CyanotisarachnoideaC. The biggest Clarke), also known as pearl grass, cockscomb, etc, is a duck toe grass blue ear grass genera perennial herbaceous plants, widely distributed in yunnan province, tonify deficiency, dehumidification, relaxes the muscles and efficacy, Tetroxone was isolated from this plant for the first time. The preparation of dew grass (2.5kg) was heated with 75% ethanol reflux extraction, ethanol recovery, concentrate. Extraction with ethyl acetate and n-butanol; The ethyl acetate extract was extracted on an Al2O3(neutral) column with gradient elution of chloroform-methanol. The eluted portion of chloroform-methanol (4∶1) was recrystallized by ethyl acetate and methanol to obtain compound 3(200mg). The eluting fraction was prepared by preparative TLC (silica gel GF254, stationary phase; Mobile phase: chloroform-methanol =5∶1) to obtain compound 1(10mg); Tetroxone (5mg) was separated from the eluted part by preparative high performance liquid chromatography (ODChemicalbookS, methanol/water =31∶ 69,242 nm). Bioactive Turkesterone was isolated from Ajugaturkestanica, Turkesterone is a potent ecdysone that is used as an ecdysterone receptor (EcR) agonist in some insect systems. Target Ecdysteroidreceptor (EcR) in vitro study TheabilityofTurkesteronetodisplace [3 h] ponAfrominvitro - expressedDmEcR/DmUSPreceptorcomplexi Sassessed. TheEC50is0. 8 mu MintheBIIbioassay Kivalueis90nMinthereceptorassay.